CSIR NET Life Science Unit 14 MCQs with Answers

Here below are 15 core Methods in Biology topics across five parts. Choose Easy, Medium or Difficult for each topic to practise 15 questions per level. Answers show the correct option and explanation immediately.

Part 1: Topics 1–3

1. Light Microscopy and Imaging

15 questions per difficulty level · Select a level

2. Fluorescence and Confocal Microscopy

15 questions per difficulty level · Select a level

3. Electron Microscopy

15 questions per difficulty level · Select a level

Part 2: Topics 4–6

4. Centrifugation and Cell Fractionation

15 questions per difficulty level · Select a level

5. Chromatographic Techniques

15 questions per difficulty level · Select a level

6. Electrophoresis and Blotting

15 questions per difficulty level · Select a level

Part 3: Topics 7–9

7. Spectroscopy and Spectrophotometry

15 questions per difficulty level · Select a level

8. Mass Spectrometry and Proteomics

15 questions per difficulty level · Select a level

9. PCR, Cloning and Sequencing

15 questions per difficulty level · Select a level

Part 4: Topics 10–12

10. Immunological and Cell Analysis Methods

15 questions per difficulty level · Select a level

11. Cell Culture and Experimental Models

15 questions per difficulty level · Select a level

12. Radioisotope and Tracer Techniques

15 questions per difficulty level · Select a level

Part 5: Topics 13–15

13. Biostatistics and Experimental Design

15 questions per difficulty level · Select a level

14. Bioinformatics and Data Analysis Methods

15 questions per difficulty level · Select a level

15. Experimental Controls and Research Ethics

15 questions per difficulty level · Select a level

Frequently Asked Questions

How does PCR amplify a specific DNA sequence?

Repeated denaturation, primer annealing and extension cycles copy a target region using a thermostable polymerase.

What is the difference between Western and Southern blotting?

Western blotting detects proteins; Southern blotting detects specific DNA sequences.

Why is a negative control important in an experiment?

It helps identify background signals, contamination or effects unrelated to the variable being tested.

How does flow cytometry analyze cells?

Cells pass individually through a laser-based detector, enabling measurement of scatter and fluorescent signals.

What is the difference between accuracy and precision?

Accuracy describes closeness to the true value; precision describes repeatability or closeness among measurements.

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