CSIR NET Life Sciences & Biotechnology: 150 Numerical and Analytical Practice Questions
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Numerical and Analytical Questions (1–150)
Unit 1: Structure and Function of Biomolecules
Q1. An enzyme converts 10 μmol substrate in 2 min. What is the average rate? (Model Sample Paper 2026)
Q2. A protein has 4 α-helices with 4 residues each. How many residues are in these helices? (Model Sample Paper 2026)
Q3. A reaction releases 22 kJ for 5 mol of product. Find energy released per mol. (Model Sample Paper 2026)
Q4. A solution contains 63 mg protein in 3 mL. Find its concentration. (Model Sample Paper 2026)
Q5. A sample contains 140 nmol ligand and 20 nmol is bound. What percentage is bound? (Model Sample Paper 2026)
Q6. An enzyme converts 15 μmol substrate in 7 min. What is the average rate? (Model Sample Paper 2026)
Q7. A protein has 9 α-helices with 5 residues each. How many residues are in these helices? (Model Sample Paper 2026)
Q8. A reaction releases 27 kJ for 6 mol of product. Find energy released per mol. (Model Sample Paper 2026)
Q9. A solution contains 68 mg protein in 4 mL. Find its concentration. (Model Sample Paper 2026)
Q10. A sample contains 190 nmol ligand and 25 nmol is bound. What percentage is bound? (Model Sample Paper 2026)
Q11. An enzyme converts 20 μmol substrate in 12 min. What is the average rate? (Model Sample Paper 2026)
Unit 2: Cellular Organization
Q12. A cell population increases from 20 to 80 cells. What is the fold increase? (Model Sample Paper 2026)
Q13. 13 vesicles each transport 3 molecules. How many molecules are transported? (Model Sample Paper 2026)
Q14. A cell has 84 chromosomes before division and distributes them equally among 4 daughter cells. How many per cell in this hypothetical distribution? (Model Sample Paper 2026)
Q15. An organelle occupies 10% of a 56 μm³ cell. Find organelle volume. (Model Sample Paper 2026)
Q16. 29 labeled particles cross a membrane in 5 s. Find average transport rate. (Model Sample Paper 2026)
Q17. A cell population increases from 25 to 125 cells. What is the fold increase? (Model Sample Paper 2026)
Q18. 18 vesicles each transport 4 molecules. How many molecules are transported? (Model Sample Paper 2026)
Q19. A cell has 94 chromosomes before division and distributes them equally among 5 daughter cells. How many per cell in this hypothetical distribution? (Model Sample Paper 2026)
Q20. An organelle occupies 11% of a 66 μm³ cell. Find organelle volume. (Model Sample Paper 2026)
Q21. 34 labeled particles cross a membrane in 6 s. Find average transport rate. (Model Sample Paper 2026)
Q22. A cell population increases from 30 to 180 cells. What is the fold increase? (Model Sample Paper 2026)
Unit 3: Fundamental Processes
Q23. An RNA polymerase synthesizes 120 nucleotides in 3 s. Find average synthesis rate. (Model Sample Paper 2026)
Q24. An open reading frame has 330 nucleotides including one stop codon. How many amino acids does it encode? (Model Sample Paper 2026)
Q25. A DNA fragment of 22 kb is cut into 2 equal fragments. What is each fragment length? (Model Sample Paper 2026)
Q26. An assay counts 11 mutations in 4 × 10³ bases. How many mutations per 1000 bases? (Model Sample Paper 2026)
Q27. A transcript has 44 exons, each of length 5 nucleotides. What is total exon length? (Model Sample Paper 2026)
Q28. An RNA polymerase synthesizes 170 nucleotides in 4 s. Find average synthesis rate. (Model Sample Paper 2026)
Q29. An open reading frame has 480 nucleotides including one stop codon. How many amino acids does it encode? (Model Sample Paper 2026)
Q30. A DNA fragment of 27 kb is cut into 3 equal fragments. What is each fragment length? (Model Sample Paper 2026)
Q31. An assay counts 16 mutations in 5 × 10³ bases. How many mutations per 1000 bases? (Model Sample Paper 2026)
Q32. A transcript has 49 exons, each of length 6 nucleotides. What is total exon length? (Model Sample Paper 2026)
Q33. An RNA polymerase synthesizes 220 nucleotides in 5 s. Find average synthesis rate. (Model Sample Paper 2026)
Unit 4: Cell Communication and Cell Signaling
Q34. Of 100 receptors, 20% are occupied. How many receptors are occupied? (Model Sample Paper 2026)
Q35. A signaling response increases from 31 units to 93 units. What is the fold change? (Model Sample Paper 2026)
Q36. A kinase phosphorylates 52 substrates in 5 min. Find the rate. (Model Sample Paper 2026)
Q37. 10% of 63 immune cells express a marker. How many cells express it? (Model Sample Paper 2026)
Q38. A second messenger concentration rises from 22 nM to 44 nM. Calculate the increase in nM. (Model Sample Paper 2026)
Q39. Of 150 receptors, 25% are occupied. How many receptors are occupied? (Model Sample Paper 2026)
Q40. A signaling response increases from 36 units to 144 units. What is the fold change? (Model Sample Paper 2026)
Q41. A kinase phosphorylates 57 substrates in 6 min. Find the rate. (Model Sample Paper 2026)
Q42. 11% of 68 immune cells express a marker. How many cells express it? (Model Sample Paper 2026)
Q43. A second messenger concentration rises from 27 nM to 81 nM. Calculate the increase in nM. (Model Sample Paper 2026)
Q44. Of 200 receptors, 30% are occupied. How many receptors are occupied? (Model Sample Paper 2026)
Unit 5: Developmental Biology
Q45. 16 embryos each have 2 blastomeres. What is the total number of blastomeres? (Model Sample Paper 2026)
Q46. Of 41 embryos, 4 develop normally. What percentage develop normally? (Model Sample Paper 2026)
Q47. A tissue length increases from 14 mm to 17 mm. What is the percentage increase? (Model Sample Paper 2026)
Q48. 33 progenitor cells undergo 5 divisions each, but each division replaces one cell with two. Assuming no death, what is the final cell count? (Model Sample Paper 2026)
Q49. A morphogen gradient decreases by 10 concentration units from an initial 54. What concentration remains? (Model Sample Paper 2026)
Q50. 21 embryos each have 3 blastomeres. What is the total number of blastomeres? (Model Sample Paper 2026)
Q51. Of 46 embryos, 5 develop normally. What percentage develop normally? (Model Sample Paper 2026)
Q52. A tissue length increases from 19 mm to 23 mm. What is the percentage increase? (Model Sample Paper 2026)
Q53. 38 progenitor cells undergo 6 divisions each, but each division replaces one cell with two. Assuming no death, what is the final cell count? (Model Sample Paper 2026)
Q54. A morphogen gradient decreases by 11 concentration units from an initial 59. What concentration remains? (Model Sample Paper 2026)
Q55. 26 embryos each have 4 blastomeres. What is the total number of blastomeres? (Model Sample Paper 2026)
Unit 6: System Physiology – Plant
Q56. A leaf fixes 24 μmol CO₂ in 4 min. Find the mean fixation rate. (Model Sample Paper 2026)
Q57. A plant takes up 61 mL water and loses 10 mL. What is net water gain? (Model Sample Paper 2026)
Q58. A plant has 42 stomata in 5 mm². Calculate stomatal density. (Model Sample Paper 2026)
Q59. A pigment absorbs 20% of 135 light units. How many units are absorbed? (Model Sample Paper 2026)
Q60. A shoot grows 29 mm in 5 days. Find mean growth rate. (Model Sample Paper 2026)
Q61. A leaf fixes 29 μmol CO₂ in 5 min. Find the mean fixation rate. (Model Sample Paper 2026)
Q62. A plant takes up 66 mL water and loses 11 mL. What is net water gain? (Model Sample Paper 2026)
Q63. A plant has 47 stomata in 6 mm². Calculate stomatal density. (Model Sample Paper 2026)
Q64. A pigment absorbs 25% of 160 light units. How many units are absorbed? (Model Sample Paper 2026)
Q65. A shoot grows 34 mm in 6 days. Find mean growth rate. (Model Sample Paper 2026)
Q66. A leaf fixes 34 μmol CO₂ in 6 min. Find the mean fixation rate. (Model Sample Paper 2026)
Unit 7: System Physiology – Animal
Q67. A heart beats 70 times per minute. How many beats occur in 5 min? (Model Sample Paper 2026)
Q68. Cardiac output is 61 mL/s for 3 seconds. Find total volume pumped. (Model Sample Paper 2026)
Q69. A patient inhales 92 L and exhales 15 L during a measured interval. What is the difference? (Model Sample Paper 2026)
Q70. Urine production is 103 mL over 4 hours. Find average rate. (Model Sample Paper 2026)
Q71. A muscle produces 84 N of force over an area of 10 cm². Find stress. (Model Sample Paper 2026)
Q72. A heart beats 75 times per minute. How many beats occur in 6 min? (Model Sample Paper 2026)
Q73. Cardiac output is 66 mL/s for 4 seconds. Find total volume pumped. (Model Sample Paper 2026)
Q74. A patient inhales 97 L and exhales 16 L during a measured interval. What is the difference? (Model Sample Paper 2026)
Q75. Urine production is 108 mL over 5 hours. Find average rate. (Model Sample Paper 2026)
Q76. A muscle produces 89 N of force over an area of 11 cm². Find stress. (Model Sample Paper 2026)
Q77. A heart beats 80 times per minute. How many beats occur in 7 min? (Model Sample Paper 2026)
Unit 8: Inheritance Biology
Q78. In 100 offspring, 20% have the recessive phenotype. How many offspring are recessive? (Model Sample Paper 2026)
Q79. 31 recombinants are observed among 62 progeny. What is recombination frequency? (Model Sample Paper 2026)
Q80. A genetic cross produces 72 offspring with a 1:3 phenotypic ratio. How many are in the minority class? (Model Sample Paper 2026)
Q81. In a sample of 63 alleles, 10 are allele A. Find A allele frequency as a percentage. (Model Sample Paper 2026)
Q82. If a genetic map distance is 5 cM per segment, what is the total for 104 identical segments? (Model Sample Paper 2026)
Q83. In 150 offspring, 25% have the recessive phenotype. How many offspring are recessive? (Model Sample Paper 2026)
Q84. 36 recombinants are observed among 108 progeny. What is recombination frequency? (Model Sample Paper 2026)
Q85. A genetic cross produces 115 offspring with a 1:4 phenotypic ratio. How many are in the minority class? (Model Sample Paper 2026)
Q86. In a sample of 68 alleles, 11 are allele A. Find A allele frequency as a percentage. (Model Sample Paper 2026)
Q87. If a genetic map distance is 6 cM per segment, what is the total for 109 identical segments? (Model Sample Paper 2026)
Q88. In 200 offspring, 30% have the recessive phenotype. How many offspring are recessive? (Model Sample Paper 2026)
Unit 9: Evolution and Diversity of Life Forms
Q89. A population contains 50 individuals across 5 species equally. Find individuals per species. (Model Sample Paper 2026)
Q90. A clade has 110 sampled taxa, of which 10% share a trait. How many taxa share it? (Model Sample Paper 2026)
Q91. A lineage has 32 observed substitutions across 3 million years. Find substitutions per million years. (Model Sample Paper 2026)
Q92. A sample has 43 individuals and 5 genera. Find mean individuals per genus. (Model Sample Paper 2026)
Q93. A fossil sample contains 84 specimens; 10 are identified as species X. What percent are species X? (Model Sample Paper 2026)
Q94. A population contains 55 individuals across 6 species equally. Find individuals per species. (Model Sample Paper 2026)
Q95. A clade has 160 sampled taxa, of which 15% share a trait. How many taxa share it? (Model Sample Paper 2026)
Q96. A lineage has 37 observed substitutions across 4 million years. Find substitutions per million years. (Model Sample Paper 2026)
Q97. A sample has 48 individuals and 6 genera. Find mean individuals per genus. (Model Sample Paper 2026)
Q98. A fossil sample contains 89 specimens; 11 are identified as species X. What percent are species X? (Model Sample Paper 2026)
Q99. A population contains 60 individuals across 7 species equally. Find individuals per species. (Model Sample Paper 2026)
Unit 10: Ecology and Behavioural Biology
Q100. A population grows from 100 to 105 individuals. What is its percentage growth? (Model Sample Paper 2026)
Q101. 81 organisms occupy 4 hectares. Find population density. (Model Sample Paper 2026)
Q102. A predator consumes 32 prey in 5 days. Find average consumption rate. (Model Sample Paper 2026)
Q103. A habitat contains 103 nests, of which 20 are occupied. Find occupancy percentage. (Model Sample Paper 2026)
Q104. 44 individuals migrate out and 5 migrate in. What is the net population change from migration? (Model Sample Paper 2026)
Q105. A population grows from 150 to 156 individuals. What is its percentage growth? (Model Sample Paper 2026)
Q106. 86 organisms occupy 5 hectares. Find population density. (Model Sample Paper 2026)
Q107. A predator consumes 37 prey in 6 days. Find average consumption rate. (Model Sample Paper 2026)
Q108. A habitat contains 108 nests, of which 21 are occupied. Find occupancy percentage. (Model Sample Paper 2026)
Q109. 49 individuals migrate out and 6 migrate in. What is the net population change from migration? (Model Sample Paper 2026)
Q110. A population grows from 200 to 207 individuals. What is its percentage growth? (Model Sample Paper 2026)
Unit 11: Bioinformatics and Computational Biology
Q111. A sequence alignment has 120 positions, of which 116 match. What is percent identity? (Model Sample Paper 2026)
Q112. A dataset contains 51 sequences, each 5 kb long. What is total sequence length? (Model Sample Paper 2026)
Q113. An algorithm processes 62 records in 3 s. Find processing throughput. (Model Sample Paper 2026)
Q114. Of 103 predicted genes, 10 are validated. What is the validation percentage? (Model Sample Paper 2026)
Q115. A sequence has 24 gaps across 4 aligned blocks. Find mean gaps per block. (Model Sample Paper 2026)
Q116. A sequence alignment has 170 positions, of which 165 match. What is percent identity? (Model Sample Paper 2026)
Q117. A dataset contains 56 sequences, each 6 kb long. What is total sequence length? (Model Sample Paper 2026)
Q118. An algorithm processes 67 records in 4 s. Find processing throughput. (Model Sample Paper 2026)
Q119. Of 108 predicted genes, 11 are validated. What is the validation percentage? (Model Sample Paper 2026)
Q120. A sequence has 29 gaps across 5 aligned blocks. Find mean gaps per block. (Model Sample Paper 2026)
Unit 12: Biochemical Engineering and Industrial Biotechnology
Q121. A bioreactor produces 100 g biomass in 5 h. Find average productivity. (Model Sample Paper 2026)
Q122. From 81 g feedstock, 20 g product is recovered. Find mass yield percentage. (Model Sample Paper 2026)
Q123. A fermenter receives 42 L medium at 4 L/hour. How long does filling take? (Model Sample Paper 2026)
Q124. A separation recovers 113 g of a product from 123 g initially present. Find recovery percentage. (Model Sample Paper 2026)
Q125. A culture produces 29 g metabolite in 5 L broth. Find titer. (Model Sample Paper 2026)
Q126. A bioreactor produces 150 g biomass in 6 h. Find average productivity. (Model Sample Paper 2026)
Q127. From 86 g feedstock, 21 g product is recovered. Find mass yield percentage. (Model Sample Paper 2026)
Q128. A fermenter receives 47 L medium at 5 L/hour. How long does filling take? (Model Sample Paper 2026)
Q129. A separation recovers 117 g of a product from 128 g initially present. Find recovery percentage. (Model Sample Paper 2026)
Q130. A culture produces 34 g metabolite in 6 L broth. Find titer. (Model Sample Paper 2026)
Unit 13: Advances in Biotechnology
Q131. Of 100 edited cells, 20 have the intended edit. What is editing efficiency? (Model Sample Paper 2026)
Q132. A library has 41 constructs, each with 4 barcodes. How many barcodes in total? (Model Sample Paper 2026)
Q133. A screening pipeline analyzes 62 samples in 3 h. Find throughput. (Model Sample Paper 2026)
Q134. A synthetic pathway yields 53 mg product; purification loses 10 mg. How much remains? (Model Sample Paper 2026)
Q135. A sequencing run obtains 34 million reads from 5 samples equally. Find mean reads per sample. (Model Sample Paper 2026)
Q136. Of 150 edited cells, 21 have the intended edit. What is editing efficiency? (Model Sample Paper 2026)
Q137. A library has 46 constructs, each with 5 barcodes. How many barcodes in total? (Model Sample Paper 2026)
Q138. A screening pipeline analyzes 67 samples in 4 h. Find throughput. (Model Sample Paper 2026)
Q139. A synthetic pathway yields 58 mg product; purification loses 11 mg. How much remains? (Model Sample Paper 2026)
Q140. A sequencing run obtains 39 million reads from 6 samples equally. Find mean reads per sample. (Model Sample Paper 2026)
Unit 14: Methods in Biology
Q141. A microscope image shows a 100 μm object as 500 μm. What is linear magnification? (Model Sample Paper 2026)
Q142. A chromatography run separates 61 mg compound into 3 equal fractions. Find mass per fraction. (Model Sample Paper 2026)
Q143. An assay tests 82 samples; 20 are positive. Find positivity percentage. (Model Sample Paper 2026)
Q144. A centrifuge processes 43 mL in 5 min. Find mean throughput. (Model Sample Paper 2026)
Q145. A spectrophotometer measures absorbance 0.54 for a sample diluted 5-fold. Assuming linearity, what is the undiluted equivalent absorbance? (Model Sample Paper 2026)
Q146. A microscope image shows a 150 μm object as 900 μm. What is linear magnification? (Model Sample Paper 2026)
Q147. A chromatography run separates 66 mg compound into 4 equal fractions. Find mass per fraction. (Model Sample Paper 2026)
Q148. An assay tests 87 samples; 21 are positive. Find positivity percentage. (Model Sample Paper 2026)
Q149. A centrifuge processes 48 mL in 6 min. Find mean throughput. (Model Sample Paper 2026)
Q150. A spectrophotometer measures absorbance 0.59 for a sample diluted 6-fold. Assuming linearity, what is the undiluted equivalent absorbance? (Model Sample Paper 2026)