Here below are 15 core Advances in Biotechnology topics across five parts. Choose Easy, Medium or Difficult for each topic to practise 15 questions per level. Answers show the correct option and explanation immediately.
Part 1: Topics 1–3
1. Recombinant DNA Technology
2. PCR and Molecular Diagnostics
3. CRISPR and Genome Editing
Part 2: Topics 4–6
4. Gene Delivery and Expression
5. Synthetic Biology
6. Stem Cells and Regenerative Medicine
Part 3: Topics 7–9
7. Gene Therapy
8. Monoclonal Antibodies and Biologics
9. Vaccines and Immunotechnology
Part 4: Topics 10–12
10. Genomics and Functional Genomics
11. Proteomics and Metabolomics
12. Single-Cell and Spatial Technologies
Part 5: Topics 13–15
13. Agricultural Biotechnology
14. Industrial and Environmental Biotechnology
15. Biosafety Ethics and Regulation
Frequently Asked Questions
How does CRISPR–Cas9 recognize a DNA target?
A guide RNA pairs with complementary target DNA adjacent to a compatible PAM sequence.
What is the difference between gene editing and transgene insertion?
Gene editing changes a genomic sequence at a targeted site; transgene insertion introduces an additional DNA sequence.
Why are monoclonal antibodies useful in biotechnology?
They recognize defined molecular targets and can be developed for diagnostic, research or therapeutic applications.
What is the purpose of a selectable marker in genetic engineering?
It helps identify cells that have acquired a desired genetic construct or modification.
How do viral vectors help deliver genetic material?
Engineered viral particles can transfer nucleic acid cargo into target cells, subject to vector-specific limitations.